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mutant ythdc1  (Addgene inc)
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Fig. 2. <t>YTHDC1</t> Knockdown Prolongs the Duration of the Early Stage of Mitosis
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Fig. 2. YTHDC1 Knockdown Prolongs the Duration of the Early Stage of Mitosis

Journal: Biological & pharmaceutical bulletin

Article Title: N 6 -Methyladenosine Reader Protein YTHDC1 Supports Proper Mitotic Progression Partly through Regulation of TPX2-Aurora A Signaling.

doi: 10.1248/bpb.b24-00542

Figure Lengend Snippet: Fig. 2. YTHDC1 Knockdown Prolongs the Duration of the Early Stage of Mitosis

Article Snippet: Doxycycline (Dox)-inducible cell lines expressing FLAG and HA-tagged wild-type or mutant YTHDC1 (A549/YTHDC1-WT or -W377A/W428A) were established as previously described.11) Plasmids pcDNA3-FLAG-HA-hYTHDC1 was a gift from Samie Jaffrey (Addgene plasmid # 85167; http://n2t.net/ addgene:85167; RRID:Addgene_85167).12) FLAG-HA epitopetagged YTHDC1 was subcloned into pENTR4-no-ccDB (686-1) [a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17424; http://n2t.net/addgene:17424; RRID: Addgene_17424)].13) The small interfering RNA (siRNA)-resistant YTHDC1 plasmid was created by introducing a silent mutation into the siYTHDC1#1 target site by PCR using the following primers: (sense) 5′-CTGGTCTCCAAGCCACTGAGCTCATCTGTTAGC-3′ and (antisense) 5′-CTGCCTCGAATGGACAGAAGGCTTTTGTCG-3′.

Techniques: Knockdown

Fig. 3. The Spindle Assembly Checkpoint Is Involved in the YTHDC1 Knockdown-Induced Delay in Mitotic Progression

Journal: Biological & pharmaceutical bulletin

Article Title: N 6 -Methyladenosine Reader Protein YTHDC1 Supports Proper Mitotic Progression Partly through Regulation of TPX2-Aurora A Signaling.

doi: 10.1248/bpb.b24-00542

Figure Lengend Snippet: Fig. 3. The Spindle Assembly Checkpoint Is Involved in the YTHDC1 Knockdown-Induced Delay in Mitotic Progression

Article Snippet: Doxycycline (Dox)-inducible cell lines expressing FLAG and HA-tagged wild-type or mutant YTHDC1 (A549/YTHDC1-WT or -W377A/W428A) were established as previously described.11) Plasmids pcDNA3-FLAG-HA-hYTHDC1 was a gift from Samie Jaffrey (Addgene plasmid # 85167; http://n2t.net/ addgene:85167; RRID:Addgene_85167).12) FLAG-HA epitopetagged YTHDC1 was subcloned into pENTR4-no-ccDB (686-1) [a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17424; http://n2t.net/addgene:17424; RRID: Addgene_17424)].13) The small interfering RNA (siRNA)-resistant YTHDC1 plasmid was created by introducing a silent mutation into the siYTHDC1#1 target site by PCR using the following primers: (sense) 5′-CTGGTCTCCAAGCCACTGAGCTCATCTGTTAGC-3′ and (antisense) 5′-CTGCCTCGAATGGACAGAAGGCTTTTGTCG-3′.

Techniques: Knockdown

Fig. 4. YTHDC1 Supports Mitotic Progression Partly through Regulation of Centrosomal Abundance of Aurora A

Journal: Biological & pharmaceutical bulletin

Article Title: N 6 -Methyladenosine Reader Protein YTHDC1 Supports Proper Mitotic Progression Partly through Regulation of TPX2-Aurora A Signaling.

doi: 10.1248/bpb.b24-00542

Figure Lengend Snippet: Fig. 4. YTHDC1 Supports Mitotic Progression Partly through Regulation of Centrosomal Abundance of Aurora A

Article Snippet: Doxycycline (Dox)-inducible cell lines expressing FLAG and HA-tagged wild-type or mutant YTHDC1 (A549/YTHDC1-WT or -W377A/W428A) were established as previously described.11) Plasmids pcDNA3-FLAG-HA-hYTHDC1 was a gift from Samie Jaffrey (Addgene plasmid # 85167; http://n2t.net/ addgene:85167; RRID:Addgene_85167).12) FLAG-HA epitopetagged YTHDC1 was subcloned into pENTR4-no-ccDB (686-1) [a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17424; http://n2t.net/addgene:17424; RRID: Addgene_17424)].13) The small interfering RNA (siRNA)-resistant YTHDC1 plasmid was created by introducing a silent mutation into the siYTHDC1#1 target site by PCR using the following primers: (sense) 5′-CTGGTCTCCAAGCCACTGAGCTCATCTGTTAGC-3′ and (antisense) 5′-CTGCCTCGAATGGACAGAAGGCTTTTGTCG-3′.

Techniques:

Fig. 5. YTHDC1 Represses the Centrosomal Abundance of Aurora A through the Regulation of TPX2 Expression

Journal: Biological & pharmaceutical bulletin

Article Title: N 6 -Methyladenosine Reader Protein YTHDC1 Supports Proper Mitotic Progression Partly through Regulation of TPX2-Aurora A Signaling.

doi: 10.1248/bpb.b24-00542

Figure Lengend Snippet: Fig. 5. YTHDC1 Represses the Centrosomal Abundance of Aurora A through the Regulation of TPX2 Expression

Article Snippet: Doxycycline (Dox)-inducible cell lines expressing FLAG and HA-tagged wild-type or mutant YTHDC1 (A549/YTHDC1-WT or -W377A/W428A) were established as previously described.11) Plasmids pcDNA3-FLAG-HA-hYTHDC1 was a gift from Samie Jaffrey (Addgene plasmid # 85167; http://n2t.net/ addgene:85167; RRID:Addgene_85167).12) FLAG-HA epitopetagged YTHDC1 was subcloned into pENTR4-no-ccDB (686-1) [a gift from Eric Campeau & Paul Kaufman (Addgene plasmid # 17424; http://n2t.net/addgene:17424; RRID: Addgene_17424)].13) The small interfering RNA (siRNA)-resistant YTHDC1 plasmid was created by introducing a silent mutation into the siYTHDC1#1 target site by PCR using the following primers: (sense) 5′-CTGGTCTCCAAGCCACTGAGCTCATCTGTTAGC-3′ and (antisense) 5′-CTGCCTCGAATGGACAGAAGGCTTTTGTCG-3′.

Techniques: Expressing